b-Galactosyl Yariv Reagent Binds to the b-1,3-Galactan of Arabinogalactan Proteins1[W][OA]
نویسندگان
چکیده
Division of Life Science, Graduate School of Science and Engineering (K.K., Y.T., T.K.), Department of Biochemistry and Molecular Biology, Faculty of Science (Y.Y.), and Institute for Environmental Science and Technology (T.K.), Saitama University, Sakura-ku, Saitama 338–8570, Japan; Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom (T.Tr., P.D.); Department of Organic and Polymeric Materials (Y.H., S.Kaw.) and Department of Applied Chemistry (H.T., T.Ta.), Graduate School of Science and Engineering, Tokyo Institute of Technology, Meguro-ku, Tokyo 152–8552, Japan; Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria (L.A.); and Food Biotechnology Division, National Food Research Institute, Tsukuba, Ibaraki 305–8642, Japan (S.Kan.)
منابع مشابه
β-galactosyl Yariv reagent binds to the β-1,3-galactan of arabinogalactan proteins.
Yariv phenylglycosides [1,3,5-tri(p-glycosyloxyphenylazo)-2,4,6-trihydroxybenzene] are a group of chemical compounds that selectively bind to arabinogalactan proteins (AGPs), a type of plant proteoglycan. Yariv phenylglycosides are widely used as cytochemical reagents to perturb the molecular functions of AGPs as well as for the detection, quantification, purification, and staining of AGPs. How...
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Arabinogalactan proteins (AGPs) are a family of highly glycosylated, hydroxyproline-rich glycoproteins implicated in various aspects of plant growth and development. (beta-D-glucosyl)3 and (beta-D-galactosyl)3 Yariv phenylglycosides, commonly known as Yariv reagents, specifically bind AGPs in a non-covalent manner. Here (beta-D-galactosyl)3 Yariv reagent was added to Arabidopsis thaliana cell s...
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Proteins decorated with arabinogalactan (AG) have important roles in cell wall structure and plant development, yet the structure and biosynthesis of this polysaccharide are poorly understood. To facilitate the analysis of biosynthetic mutants, water-extractable arabinogalactan proteins (AGPs) were isolated from the leaves of Arabidopsis (Arabidopsis thaliana) plants and the structure of the AG...
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The cortical array of microtubules inside the cell and arabinogalactan proteins on the external surface of the cell are each implicated in plant morphogenesis. To determine whether the cortical array is influenced by arabinogalactan proteins, we first treated Arabidopsis roots with a Yariv reagent that binds arabinogalactan proteins. Cortical microtubules were markedly disorganized by 1 microM ...
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